$VAR1 = 'page'; $VAR2 = 'mainindex'; $VAR3 = 'dbase'; $VAR4 = 'optimized'; $VAR5 = 'file'; $VAR6 = 'bcereusgrp_isolates.xml'; Content-Type: text/html; charset=ISO-8859-1 Bacillus cereus group Tourasse-Helgason MLST Database

Bacillus cereus group MLST Database (Tourasse-Helgason scheme)

LATEST NEWS (July 2020):
   MAJOR UPDATE OF THE DATABASE: ~250 additional isolates, most of them with complete genomes.
   MLST sequences and information from 19 brazilian B. cereus and B. thuringiensis isolates kindly provided by Dr. Adriana M. Vivoni, Oswaldo Cruz Institute (IOC-FIOCRUZ), Rio de Janeiro, Brazil.
   MLST sequences and information from the chinese rice isolate B. cereus 192 kindly submitted by Dr. Yang Yong, Zhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), Hangzhou, Zhejiang, China.
   Now including data from four recently defined species that may be novel members of the B. cereus group: B. toyonensis (Jimenez et al. 2013), B. gaemokensis (Jung et al. 2010), B. manliponensis (Jung et al. 2011), and B. bingmayongensis (Liu et al. 2014).

View previous news here.

TH Database Info

   This database provides information and sequence data for all isolates that have been typed using the MLST method based on the combined scheme of Tourasse, Helgason et al. 2006, as well as strains whose complete genomes have been sequenced (currently 2106). Click the MLST Scheme link for detailed information about the scheme and click the Primers and Protocol link for the gene amplification and sequencing protocol. The whole database currently contains data for 313 isolates representing 175 sequence types (STs), as of August 1, 2019. The Strain Statistics page gives overall statistics about species distribution and strain origin.
   Conflicting isolates: Note that an additional set of 0 isolates, including 0 of the complete genomes, have not been included in the main database because they exhibit conflicting phylogenetic positions among MLST schemes and/or typing methods, or they exhibit conflicting typing data from different collections or databases. These conflicting isolates are listed here.

   The isolates have been classified according to the seven major phylogenetic clusters of the B. cereus group population defined by Guinebretiere et al. 2008 (denoted I-VII). In order to provide a classification that is consistent across typing methods, isolates have been assigned to a particular cluster if they belong to that cluster in MLST-only, MLST+AFLP, and MLST+AFLP+MLEE phylogenetic analyses. Otherwise, isolates that could not be assigned consistently to a given cluster or that do not belong to any of the seven major clusters are classified as "unclustered". The branches of every phylogenetic tree provided or generated by the database are color-coded according to cluster. An additional tool for affiliating isolates to the seven clusters based on the panC gene sequence is provided by Guinebretiere et al. at this URL (see Guinebretiere et al. 2010).

   The species assignments listed in the database correspond to the original assignments reported in publications, bacterial culture collections, and sequence databases. Some isolates may have to be reclassified upon further phylogenetic, genetic, and/or microbiological studies.

Note: The MLST data of the Tourasse-Helgason scheme have also been combined with data from the 4 other MLST schemes available for the B. cereus group in the multi-scheme SuperCAT database, and with typing data from Amplified Fragment Length Polymorphism (AFLP) and MultiLocus Enzyme Electrophoresis (MLEE) in the multi-datatype HyperCAT database, also provided on this server.